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Molecular Structure of Melanocortin receptors and their accessory proteins

Funding: Latvian Council of Science

Project number: 364/2012

Period: 2013-2016

Project manager: Dr.biol.D.Fridmanis

GPCR family is the largest group of membrane receptors. They are the targets for 40% of modern drugs, thus solving their molecular structure is crucial. Protein X-ray crystallography is the method of choice for determination of large protein molecular structure. ACTHR is one of the five MCRs, which are regulating multitude of vital functions. Among other MCRs ACTHR stands out with amazing specificity, it binds only ACTH and is transported to cell membrane only in the presence of MRAP. The aim of the this study is to determine the molecular structure of ACTHR and ACTHR–MRAP complex. To reach this goal we shall employ Baculovirus expression system for production, affinity chromatography for purification, lipidic cubic phases for crystallization and x-ray crystallography for structure determination. The results of this study shall broaden the understanding on molecular aspects of GPCR action and have a potential of being employed in generation of novel pharmaceutical compounds.

Mājas lapas izstrādi finansēja ERAF aktivitātes projekts Nr. 2010/0196/2DP/ "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".