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Investigation of interaction of smoked dietary products with gut microbiome

 

Funding: European Regional Development Fund (ERDF) “On Implementation of Activity 1.1.1.2 “Post-doctoral Research Aid” of the Specific Aid Objective 1.1.1 “To increase the research and innovative capacity of scientific institutions of Latvia and the ability to attract external financing, investing in human resources and infrastructure” of the Operational Programme “Growth and Employment”

Project Title:Investigation of interaction of smoked dietary products with gut microbiome

Project Nr.: 1.1.1.2/VIAA/1/16/128

Period: 36 month (1st January 2018 – 31 st December 2020)

Project costs: 133 806,00 EUR

Project implementer: Dr.biol. I. Kalniņa

Considering the immense choice of harboured enzymes the gut microbiome is a serious contributor to biotransformation of incoming compounds with a prominent role in health and disease. Wood smoke used in processing of food products for flavouring and preservation contains a great variety of compounds with potential to interact with the microbiome. The main objective of the project will be multidimensional investigation of the impact smoked food especially fish consumption exerts on human gut microbiome to gain insight in the responding functional pathways and metabolic consequences. The study will be designed as the crossover dietary intervention including following experimental steps: evaluation of taxonomic and functional composition of the microbiome in humans, detailed characterisation of distinct target communities using gnotobiotic mice, investigation of impact of smoke compounds on microbial communities residing in distinct sections of the gut. Implementation of the proposed project will give insight in the mutual interaction of bacterial taxa and the role individual members play in the community, as well as add mechanistic understanding on "microbiome to host" metabolic crosstalk. Answering these questions will contribute to the data pool necessary for designing microbiome based tools and consultation on dietary choice to improve healthcare.

Information published 02.01.2018.

 

Progress of the project

1 January 2018 - 31 March 2018

 The project is aimed at investigation of the impact of smoked food consumption on the human gut microbiome. It is expected that the crossover dietary intervention study will provide novel information not only on the response of gut microbiome to the smoked foods, but also wide spectrum of other food items commonly included in daily diet. During the first quarter of the project implementation study specific informed consent forms and questionnaires for diet and lifestyle related information collection have been elaborated. The project protocol has been submitted for consideration to the Central Medical Ethics Committee. Currently planning of the dietary intervention stage and survey of potential participants is in progress.

Information published 29.03.2018.

 

Progress of the project

1 April 2018 – 30 June 2018

For evaluation of impact increased smoked food consumption has on the human gut microbiome 26 participants have been recruited to dietary intervention study. Two participants have withdrawn from the study during the second week due to the factors incompatible with requirements of the study. The study is designed to contain three week-long intervention periods concurrently covering all types of target diet (fish, smoked fish and smoked ham) intervened by washout periods two weeks each. Dietary intervention phase of the project will be completed by the end of the august. General phenotypic information from the participants who continue the intervention has been collected using LGDB and project specific questionnaires comprising questions on medication, diet as well as other lifestyle factors. Background lifestyle factors during the intervention are controlled using standardized inquiry forms filled up throughout the study. The study protocol has been approved by Central Medical Ethics Committee of Latvia (Nr. 1/18-06-07).

Information published 29.06.2018.

 

Progress of the project

1 July 2018 – 30 September 2018

Recently dietary intervention stage of the project has been completed. In total 24 participants aged 19 - 62 years (average age 39,5 years) were involved in 11 weeks long study comprising three week-long intervention periods concurrently covering three types of target diet.  The target diet included fish (salmon provided raw and cooked at home), smoked salmon and smoked pork (in both cases smoked with hardwood smoke). During the study participants monitored their daily diet as well as reported used food supplements and medicaments. Blood samples were collected before and after each intervention period for biochemical analysis (complete blood count, lipids and inflammatory markers) and participants donated stool samples for the gut microbiome analysis. Currently samples are under processing for the gut microbiome profiling including high throughput sequencing analysis.

Information published 28.09.2018.




Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".