Project title: Enhanced hollow fiber bioreactor add-on for induced therapeutic EV production (EVBoost)

Project No.: OSI_PIP_BioPhoT-2025/1-0030

Period: 1 October 2025 – 30 September 2025

Project costs: 200 000,00

Principal Investigator: Dr. biol. Karīna Narbute

Project summary:

The EVBoost project revolutionizes extracellular vesicle (EV) production from mesenchymal stem cells (MSCs) by introducing an innovative bioreactor add-on that uses Extremely Low Frequency Electromagnetic Field (ELF-EMF) stimulation. This addresses the critical need for scalable, cost-effective therapeutic MSC-derived EVs, a current bottleneck due to labor-intensive, low-yield methods that often compromise EV integrity. EVBoost’s novel approach non-invasively boosts EV secretion within a hollow fiber bioreactor, building on preliminary data and existing research showing enhanced EV yield without harming cell viability or EV therapeutic properties.

To achieve its goals, the project will conduct in-depth B2B market research with bioreactor manufacturers and EV therapy developers to pinpoint industry needs and customer problems. It will also secure intellectual property through a patent application with a specialized IP firm. Comprehensive multiomics analysis of EVs and cells will precisely quantify ELF-EMF’s effect on EV composition, ensuring therapeutic efficacy and validating production claims. EVBoost will engage in targeted marketing and public relations, including participation in key industry events and direct stakeholder meetings, and develop a robust business model with at least three distinct go-to-market scenarios. Finally, a detailed strategy for further research, development, and securing future financing will ensure long-term sustainability.

Information published 01.10.2025.