Project title: Quantitative Biosensing in Organ-on-Chip Systems (Q-BIOS)

Project No.: lzp-2024/1-0329

Period: 1st January 2025 – 31 December 2027

Project costs: 300 000,00 EUR

Principial Investigator: Institute of Solid State Physics, University of Latvia

Collaboration partner: Latvian Biomedical Research and Study Centre

Principal Investigator BMC: Dr. biol. Ralfs Buks

Project summary:

The aim of this project is to investigate real-time trans-epithelial electrical resistance (TEER) parameters, correlating resistance measurements in a gut-on-a-chip model with biological markers of barrier quality. Current TEER sensors lack a direct correlation with tight junction protein data, barrier integrity, and cell coverage in organ-on-a-chip systems, limiting their effectiveness. The project will assess the impact of electrode material, placement, and sampling frequency on signal-to-noise ratio and cell viability. The final experimental phase will correlate barrier integrity data with TEER measurements in the gut-on-a-chip model, accounting for oxygen load between the epithelial and endothelial barriers. The project’s significant impact lies in its thorough exploration of TEER application in gut-on-a-chip models, which could advance the adoption of this technology in other organ models. Additionally, it will provide a framework for correlating various TEER measurement approaches. Expected outcomes include three high-impact publications, six conference presentations, and a significant contribution to a doctoral thesis upon the project’s completion.

Information published 02.01.2025.