Project title: Immunomodulating properties of bacteriophage derived dsRNA of different size and their use as vaccine adjuvants

Partners:

National Cancer Institute, Center of Scientific Resesarch (NCI)

Institute of Biomedical Sciences, Academia Sinica (IBMS, AS)

Period: 2016 -2018

Principle Investigator BMC: Dr.biol. Dace Pjanova

Project Total costs: 25 000 USD

The main objectives of the proposed project are:

1. to get dsRNA molecules of different size from heterogeneous population of molecules obtained from bacteriophage-infected coli cells and select the most effectives for further investigation in term of their cytokine production;
2. to characterise the selected dsRNA molecules in term of their antiviral properties both in cell based assays and mouse models;
3. to test the in vivo vaccine adjuvant effect in mouse models;
4. to characterise the selected dsRNA molecules in term of immune modulatory properties both in mouse models and ex vivo cultivated human PBMC;
5. to perform the transcriptional analysis of PBMC after stimulation with selected dsRNA.