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LATVIAN

BIOMEDICAL

RESEARCH AND STUDY CENTRE


RESEARCH AND EDUCATION IN BIOMEDICINE FROM GENES TO HUMAN

CELL BIOLOGY AND MICROSCOPY CORE FACILITY

Human and animal cell culturing has become an integrated part of biomedical and biotechnology research. The Cell biology core facility is the part of Biomedical technology complex at BMC that joins the knowledge, skills, and expertise of research personnel as well as gives centralised access to broad array of equipment. The core facility ensures mammalian cell culture maintenance, characterization and functional testing and the obtaining of primary cell cultures. All cell culture boxes are equipped with automated cell counters LunaFX8.

 The cell culturing area is designed and equipped according to the biosafety level 2. There are three separated blocks for the work with (1) certified cell lines (i.e., obtained from ATCC, ECACC etc.), (2) clinical material to obtain primary cell cultures/lines, and (3) cell lines infected with non-pathogenic viruses or virus-like particles. The main cell culture area is accompanied with a support room for washing and sterilization, several additional microscopy rooms and a biochemistry room. The facility ensures short-term and long-term storage of cells at -80oC and liquid nitrogen tanks.

 The core facility has the capacity of six working places within the cell culture rooms, three at fluorescent microscopes; biochemistry room holds a place for up to five people. Under the supervision of qualified specialists, personnel training for golden standards of cell culturing and characterization is ensured; special attention is paid to the student training. To ensure optimal use of the equipment, it is operated by designated BMC personnel.

The video of the Latvian Biomedical Research and Study Centre Biomedical Technology complex

 

Ramona Petrovska

Ramona Petrovska

Head of the Cell biology and microscopy core facility, researcher

Personāls

Ramona Petrovska , MSc. biol.

Head of Cell biology core facility, researcher
Phone: +371 28226298
E-mail: ramona@biomed.lu.lv

Cell culture bank establishment, student training

 

Juris Jansons , Dr. biol.

Researcher
Phone: +371 67808212
E-mail: jansons@biomed.lu.lv

Responsible for FC/FACS, ImmunoSpot CTL, electronmicroscope

 

Ilona Mandrika , MSc. biol.

Researcher
Phone: +371 67808200
E-mail: ilona@biomed.lu.lv

Work with a live cell visualization system

Ance Roga, MSc. biol.

E-mail: ance.roga@biomed.lu.lv

Phone: +371 67808200

Work with a confocal microscope

 

Fēlikss Rūmnieks, MSc. biol.

E-mail: felikss.rumnieks@biomed.lu.lv

Phone: +371 67808200

Work with a confocal microscope

 

Justs Jāzeps Zaļums Bsc. biol.

E-pasts: justs.zalums@biomed.lu.lv

Telefons: +371 67808200

Working with a live cell visualization system

Provided services

  • Cell culturing
  • Obtaining of primary cell cultures/lines
  • Cell line bank & cell culture storage
  • Student training for cell culturing & testing
  • Routine testing for mycoplasma contamination
  • Cell counting & sorting (FACSAria, BD, CYTEC Aurora, CYTEC AuroraCS)
  • Fluorescent microscopy (LEICA DMI 3000 B, LEICA DM 3000 un LEICA TCS SP2 SE)
  • ELISPOT analyses (ImmunoSpot CTL)
  • Microplate reader Tecan Infinite F200
  • Automated cell counter CellDrop FL (DeNovix)
  • Biochemistry room for cell & tissue sample characterization
  • Laser scanning confocal microscope LEICA TCS SP8 (visualization of live and fixed cells, monitoring of cells innormoxy/hypoxia conditions