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2019

Latvian Biomedical Research and Studies Centre launches new project "RNA molecular determinants in development of pituitary adenoma”


 

 

On 1st April BMC will be launched European Regional Development Fund (ERDF) Operational Programme Growth and Employment 1.1.1 Specific Aid Objective to increase the research and innovative capacity of scientific institutions of Latvia and the ability to attract external financing, investing in human resources and infrastructure Measure 1.1.1.1 “Support for applied research” 2. Selection round.

"RNA molecular determinants in development of pituitary adenoma” (Project Identification No 1.1.1.1/18/A/089). The project principle investigator in BMC is Dr. biol. Vita Rovīte. Cooperation partner is SIA “GenEra”.

About the project:

The scientific objective of the project is to study the spectrum of various ribonucleic acid (RNA) markers (mRNA, non-coding) in development of pituitary adenoma (PA) to discover determinants that influence therapy response and disease outcome.

The main activities to achieve the goal of the project: 1) identify and analyse molecular RNA factors in primary PA tissue by massive parallel sequencing (MPS), 2) study RNA types in primary culture of free-floating spheres and mesenchymal cells from PA tissue to describe tumour-borne RNA patterns, 3) investigate alterations in coding and non-coding RNA in free-floating sphere model upon addition of PA treatment medication, 4) validate discovered markers in PA patients’ plasma and independent cohort, 5) functionally characterize discovered RNA candidates in GH3 cells.

The results of the project will evaluate molecular RNA factors on several functional levels of PA derived biological materials and models: (1) potential makers discovered in tissues and validated in plasma and/or tissues could serve for prediction of disease outcome, characterization of aggressiveness and non-invasive diagnostics, (2) study of free-floating spheres and mesenchymal cells will help to estimate spheres as isolated model for PA tumour-borne cells, define their RNA profile and describe PA therapy response in this system, (3) research of influence of discovered RNA candidates in GH3 cells will functionally characterize the precise cellular mechanisms of these factors in PA development.

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Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".