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The development of an efficient pilot-scale leghemoglobine production technology, based on recombinant Pichia pastoris and Kluyveromyceslactis fed-batch fermentations (BioHeme)

 

 

Project Title: „The development of an efficient pilot-scale leghemoglobine production technology, based on recombinant Pichia pastoris and Kluyveromyceslactis fed-batch fermentations (BioHeme)”

Funding: European Regional Development Fund (ERDF), Measure 1.1.1.1 “Support for applied research”

Project No.: 1.1.1.1/21/A/044

Period: 1 January 2022 – 30 November 2023

Project costs: 540 540,00 EUR

Project implementer: Latvian State Institute of Wood Chemistry 

Cooperation partner: Latvian Biomedical Research and Study Centre

Principle Investigator BMC: Dr.biol. A. Kazāks

 

Project summary:

An alternative to livestock production can be plant-based meat. Plant-based meat is defined as a meat-like substance made from vegetarian friendly ingredients. Plant-based meat reproduces the flavour and texture of real meat without using products of animal origin. In plant-based meats, the leghemoglobin protein (LegH) is essential to comprise the unique flavour and aroma of traditional meat. As of now, the most perspective process of manufacturing LegH in appropriate amounts, is through fermentation using recombinant microorganism strains defined as methylotrophic yeast (for example, Pichiapastoris), which utilize methanol as substrate, and in their recombinant form can produce LegH via methanol-inducible promoter element. The mentioned research is driven by two problems, which are: (1) the use of volatile and flammable substances during the production process and (2) relatively high cost of applicable substrates. One of biological platforms, which was not studied earlier for the production of LegH and can potentially outperform P.pastoris is Kluyveromyces lactis. The mentioned organism can grow on cheap substrates, such as cheese whey, and does not require the use of methanol for protein synthesis induction. Therefore, the objective of the BioHeme project: is to develop effective fed-batch fermentation and scale-up techniques for the production of plant-based meat substitutes, utilizing a recombinant LegH producing strain of Pichia pastoris and Kluyveromyceslactis. The main activities of the BioHeme project are: (i) obtainment of recombinant (LegH producing) strains of P. Pastoris and K. lactis, with a subsequent evaluation of heme production efficiency and technological potential, (ii) optimization of fermentation medium composition for biomass growth and hemeprotein production phases for the two mentioned recombinant strains, (iii) the application of Model-Predictive control systems for the control of biomass growth rates and hemeprotein synthesis, (iv) fermentation process transfer to pilot scale and evaluation of applications sustainability.

Information published 03.01.2022.



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".