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StructuralandfunctionalstudiesofLymediseaseagentBorreliaburgdorferioutersurface proteīns to revealthemechanismsofpathogenesiswiththeintention to create a newvaccine

 

 

Funding: EuropeanRegionalDevelopmentFund (ERDF)“OnImplementationofActivity 1.1.1.2 “Post-doctoralResearchAid” oftheSpecificAidObjective 1.1.1 “To increasetheresearchandinnovativecapacityofscientificinstitutionsofLatviaandtheability to attractexternalfinancing, investinginhumanresourcesandinfrastructure” oftheOperationalProgramme “GrowthandEmployment”

Project Title:

 „StructuralandfunctionalstudiesofLymediseaseagentBorreliaburgdorferioutersurface proteīns to revealthemechanismsofpathogenesiswiththeintention to create a newvaccine

Project Nr.:1.1.1.2/VIAA/1/16/144

Period:36month (1st September 2017–31stAugust 2020)

Projectcosts:133 806,00 EUR

Projectimplementer: Dr.biol. K. Brangulis

 

The goal of this Project is to solve the 3-d structures of Lyme disease agent B. burgdorferi outer surface proteins predicted to be important in the pathogenesis of Lyme disease to reveal the molecular details of action that could be used in future to design new strategies to stop the infection.

Objectives and overview of the action – to select the target B. burgdorferi outer surface proteins and to make expression vector constructs. The selection criteria for the target proteins will be based on the importance in the pathogenesis of Lyme disease as judged from the scientific articles.

However, it must be emphasized that for most of the up-regulated B. burgdorferi outer surface proteins known to be potentially important in the pathogenesis of Lyme disease, the exact function/ligand/receptor is unknown, and they do not possess any homology with proteins in any other organism whose genome has been sequenced, that also prevents to make any assumption about the potential function. Therefore a comprehensive study of outer surface proteins is required to reveal the molecular details of pathogenesis of Lyme disease which remains poorly understood. It will certainly provide a significant contribution in the understanding of the molecular details of Lyme disease action but most importantly is that the acquired knowledge about the molecular details of outer surface proteins is intended to be used to develop new strategies to fight against the Lyme disease in humans.

Information published: 01.09.2017.

 

Progress of the project

1 September 2017 - 30 November 2017

In the current period the project was carried out according to the project time diagramma, i.e., the research has been dedicated to perform according to work packages 1 and 2 (WP1 and WP2). WP1 and WP2 include the selection of target Borrelia burgdorferi outer surface proteins for design of expression constructs and production of proteins in Escherichia coli cells and purification of expressed borrelial proteins.

In the period until 30.11.2017 15 different B. burgdorferi outer surface proteins which have showed some role in the pathogenesis of Lyme disease have been selected. 15 different expression vector constructs for protein expression in E. coli have been made accordingly. 5 different proteins have been expressed in high quantities, cells have been sonicated and all 5 proteins have been purified using chromatography methods. Crystallization trials have been started for 2 proteins.

Information published: 30.11.2017.



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".