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RNA molecular determinants in development of pituitary adenoma

Project Title: „RNA molecular determinants in development of pituitary adenoma”

Funding: European Regional Development Fund (ERDF), Measure “Support for applied research” 

Project No.:

Period: 1 April 2019 – 31 March 2022

Project costs: 648 648 EUR

Principle Investigator: Dr. biol. Vita Rovīte

Cooperation partner: SIA “GenEra” 


Project summary:

The scientific objective of the project is to study the spectrum of various ribonucleic acid (RNA) markers (mRNA, non-coding) in development of pituitary adenoma (PA) to discover determinants that influence therapy response and disease outcome.

The main activities to achieve the goal of the project: 1) identify and analyse molecular RNA factors in primary PA tissue by massive parallel sequencing (MPS), 2) study RNA types in primary culture of free-floating spheres and mesenchymal cells from PA tissue to describe tumour-borne RNA patterns, 3) investigate alterations in coding and non-coding RNA in free-floating sphere model upon addition of PA treatment medication, 4) validate discovered markers in PA patients’ plasma and independent cohort, 5) functionally characterize discovered RNA candidates in GH3 cells.

The results of the project will evaluate molecular RNA factors on several functional levels of PA derived biological materials and models: (1) potential makers discovered in tissues and validated in plasma and/or tissues could serve for prediction of disease outcome, characterization of aggressiveness and non-invasive diagnostics, (2) study of free-floating spheres and mesenchymal cells will help to estimate spheres as isolated model for PA tumour-borne cells, define their RNA profile and describe PA therapy response in this system, (3) research of influence of discovered RNA candidates in GH3 cells will functionally characterize the precise cellular mechanisms of these factors in PA development.

Information published 01.04.2019.

Mājas lapas izstrādi finansēja ERAF aktivitātes projekts Nr. 2010/0196/2DP/ "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".