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RNA molecular determinants in development of pituitary adenoma

Project Title: „RNA molecular determinants in development of pituitary adenoma”

Funding: European Regional Development Fund (ERDF), Measure 1.1.1.1 “Support for applied research” 

Project No.: 1.1.1.1/18/A/089

Period: 1 April 2019 – 31 March 2022

Project costs: 648 648 EUR

Principle Investigator: Dr. biol. Vita Rovīte

Cooperation partner: SIA “GenEra” 

 

Project summary:

The scientific objective of the project is to study the spectrum of various ribonucleic acid (RNA) markers (mRNA, non-coding) in development of pituitary adenoma (PA) to discover determinants that influence therapy response and disease outcome.

The main activities to achieve the goal of the project: 1) identify and analyse molecular RNA factors in primary PA tissue by massive parallel sequencing (MPS), 2) study RNA types in primary culture of free-floating spheres and mesenchymal cells from PA tissue to describe tumour-borne RNA patterns, 3) investigate alterations in coding and non-coding RNA in free-floating sphere model upon addition of PA treatment medication, 4) validate discovered markers in PA patients’ plasma and independent cohort, 5) functionally characterize discovered RNA candidates in GH3 cells.

The results of the project will evaluate molecular RNA factors on several functional levels of PA derived biological materials and models: (1) potential makers discovered in tissues and validated in plasma and/or tissues could serve for prediction of disease outcome, characterization of aggressiveness and non-invasive diagnostics, (2) study of free-floating spheres and mesenchymal cells will help to estimate spheres as isolated model for PA tumour-borne cells, define their RNA profile and describe PA therapy response in this system, (3) research of influence of discovered RNA candidates in GH3 cells will functionally characterize the precise cellular mechanisms of these factors in PA development.

Information published 01.04.2019.

 

Progress of the project:

1 April 2019 – 30 June 2019

The project specific sample database has been created, data was gathered about tissue from surgery material, phenotypic information of the patients has been gathered, sorted and quality controlled. DNA and RNA from newly acquired tissue samples have been carried out, and list of samples for further RNA sequencing analysis was created. The cell lines of mesenchymal cells and spheres from newly acquired primary adenoma tissue have been established and cell-derived RNA materials have been obtained for further project implementation tasks.

Information published 28.06.2019.

 

Progress of the project:

1 July 2019 – 30 September 2019

The creation of project specific sample database has been continued, data was gathered about tissue from surgery material, phenotypic information of the patients has been gathered, sorted and quality controlled. DNA and RNA from newly acquired tissue samples have been carried out, and first RNA sequencing of PA tissue have been performed. The cell lines of mesenchymal cells and spheres from newly acquired primary adenoma tissue have been established and incubated with octreotide and cabergoline, cell-derived RNA materials have been obtained for further project implementation tasks.

Information published 30.09.2019.




Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".