Research Projects All Projects
Projects

Quick links

Diagnostic and immunoprotective potential of influenza virus hemagglutinin stalk peptide: generation of novel vaccine prototypes

 

 

Project Title:Diagnostic and immunoprotective potential of influenza virus hemagglutinin stalk peptide: generation of novel vaccine prototypes

Funding: European Regional Development Fund (ERDF), Measure 1.1.1.1 “Industry-Driven Research”

Project Nr.: 1.1.1.1/16/A/054

Period: 1st April 2017 – 31st March 2020

Project costs: 648 643,69 EUR

Principle Investigator: Dr. biol. A. Kazāks

 

The lack of universal influenza vaccine is a global health problem. Novadays, a special interest is attended to structurally conserved protein domains capable to elicit protection against broad range of influenza viruses. Hemagglutinin (HA) is a homotrimeric viral major surface protein which is the main target of virusneitralizing antibodies. A membrane-proximal HA stalk domain is structurally highly conservative which allow to consider it as a promising universal vaccine candidate. However, seasonal influenza vaccine or natural virus infection leads to very weak stalk-specific antibody response. A well-known strategy to improve immune response is exposition of peptides on the surface of virus-like particles (VLPs). One of the most conservative HA stalk region is so called large antigenic helix (LAH). Our recent study has revealed that this 57 aa-long peptide in E.coli forms stable α-helical trimer which is relatively easy to purify. First, project aims to generate a novel broad protection range vaccine prototype by exposition of LAH trimer on VLP surface, which is achievable by genetic fusion of the LAH gene with the coat protein gene from T=3 symmetry-derived virus. Secondly, LAH trimer will be used as antigen to develop a serodiagnostic assay for identification of people with increased anti-stalk antibody titers.

Information published: 03.04.2017.



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".