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Development of orphan G-protein coupled receptor peptidic ligand identification system

 

 

Project Title: „Development of orphan G-protein coupled receptor peptidic ligand identification system” 

Funding: European Regional Development Fund (ERDF), Measure 1.1.1.1 “Industry-Driven Research”

Project Nr.: 1.1.1.1/16/A/055

Period: 1st January 2017 – 31st December 2019

Project costs: 285 245,84 EUR

Principle Investigator: Dr. biol. D. Fridmanis

 

The aim of this Project is to develop humanized yeast S.cerevisiae based GPCR peptide ligand identification system and to evaluate its effectiveness by de-orphanization of C.intestinalis orexin-like receptor and determination of novel ligand’s pharmacological properties. The results of this Project shall provide instrument for the development of novel pharmaceutical compounds and promote development of biopharmaceutical industry in Latvia

 

Progress of the project

1 January 2017 - 31 March 2017

Experimental development

According to plan the first three months of the project were devoted to creation of double expression plasmid. Since p426GPD and p426TEF expression plasmids that have been successfully employed by our group are nearly identical, then within the scope of these activities we altered the multi-clonal sites of the expression loci in a way that each would contain only two unique cloning/restriction sites, thus becoming mutually compatible. Following the modification of both plasmids we performed the sequencing analysis of both expression loci to ascertain the conformity of acquired sequences to planned ones and carried out the transfer of TEF containing locus from p426TEF to p426GPD plasmid. At the moment we are performing the sequencing analysis of thus acquired construct.

Information published: 31.03.2017.



Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".