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Clinical utility of extracellular vesicle-enclosed human and microbial transcriptomes

 

 

Project Title: „Clinical utility of extracellular vesicle-enclosed human and microbial transcriptomes”

Funding: European Regional Development Fund (ERDF), Measure 1.1.1.1 “Support for applied research” 

Project No.: 1.1.1.1/18/A/084

Period: 1 April 2019 – 31 March 2022

Project costs: 648 648 EUR

Principle Investigator: Dr. biol. Aija Linē

Cooperation partner: SIA “GenEra” 

 

Project summary:

Extracellular vesicles (EVs) circulating in human blood are sampled from various cell types in the body, including cancer. EVs isolated from cancer patients’ blood have been shown to contain cancer-specific molecules, therefore they may reveal the presence of cancer, help to predict its behaviour and track the progression of cancer. This project is based on the results of our previous studies showing that EVs contain not only various classes of RNAs derived from human cells but also RNA fragments of microbial origin. The main aim of this project is to gain new knowledge about the composition and origin of RNA cargo of EVs circulating in cancer patients’ blood, and to assess the clinical utility of EV-enclosed human and microbial RNAs for the diagnosis, prognosis and tracking disease progression in three distinct types of cancer – prostate, breast and gastric cancer.

We plan to expand and update the longitudinal Cancer Biobank comprising detailed clinical information, tumour tissue specimens, stool and blood samples from cancer patients. EVs will be isolated from plasma and stool samples of cancer patients and cancer-free controls and their RNA content will be sequenced together with small RNAs from matched tumour and normal tissues. Multiple data analyses will be performed to assess the correlation of EV-enclosed RNAs with various clinical and pathological features and to assess their diagnostic and prognostic value. Finally, digital PCR assays for selected biomarkers will be established and used to assess their clinical utility.

We expect, this project will lead to the discovery of novel RNA biomarkers of human and microbial origin that are associated with the presence, progression or drug response of cancer and are suitable for the development of blood-based tests. Currently, this technology is at TRL2 and we expect that at the end of the project it will reach TRL4.

Information published 01.04.2019.

 

Progress of the project:

1 April 2019 – 30 June 2019

-        Development of a cohort of healthy controls is in progress;

-        An application for the permission for testing EV biomarkers in the clinical samples of prostate cancer patients has been prepared and submitted to CMEK;

-        Isolation of EVs from plasma samples has been started;

-        Optimisation of the protocol for the construction and sequencing of small RNA libraries has been started;

-        Development of the protocol for the isolation of EVs from fecal samples is in progress.

Information published 28.06.2019.




Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".