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Pauls Pumpēns , Dr. habil. biol.
E-mail:
paul@biomed.lu.lv
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Pauls
Projects
Fine functional structure of hepatitis B core particles: stable expression in eukaryotic cell lines, purification, structural peculiarities, and immunological evaluation
Vector-borne Risks for Europe: Risk assessment and control of West Nile and Chikungunya virus (VECTORIE)
Molecular epidemiology of hepatitis B anc C viruses for the development of individualised therapeutic vaccines
The molecular effects of quantitative transcriptiion factor alterations in cancer cells in vitro
Introduction of molecular pinciples in viral hepatitis diagnostics, prognostics, treatment and prevalence restriction
Artificial virus-like particles as a platform for novel vaccine, gene therapy and diagnostic tools
Novel chimeric vaccine prototypes: West nile virus
Ration design and standardized evalution of novel genetic vaccines using West Nile virus as a paradigm
Construction of melanoma chimeric vaccine candidates
Combined immune and gene therapy for chronic hepatitis
Molecular mechanisms of genetic variability and persistence of viral hepatitis
Malaria vaccine
Novel VLP model: expression and X-ray structure of coat protein and chimeras of Acinetobacter RNA phage AP205
Hematological malignancies in children and clinical properties in influence of combined chronical viral infection
New generation diagnostic of sexually transmitted diseases
Artificial chimeric proteins as diagnostic, vaccine, and gene therapy
Molecular monitoring and pathological role of HCV, HGV and altered HBV genomes in Baltic region
Bivalent hantavirus vaccine for Europe
Universal Cloning and Expression System by Means of Protease Mutants
Spatial Structure of Chimeric Capsids Based on RNA Phage Coats
Construction of E.coli Mutants - Overproducers of Chimeric Proteins RNA Phage Coats
Molecular Monitoring of HBV and HCV Infection in Latvia: Genomic Variations of HBV and HCV in Presence and Absence of HIV Infection
Publications
International
The True Story and Advantages of RNA Phage Capsids as Nanotools P Pumpēns, R Renhofa, A Dišlers, T Kozlovska, V Ose-Klinklāva, Pushko, P., K Tārs, E Grēns, Bachmann, M.F. , Intervirology, pp. 74-110 , 2016
The true story and advantages of the famous Hepatitis B virus core particles: Outlook 2016 P Pumpēns, E Grēns , Molecular Biology, 50 (4), pp. 489-509 , 2016
Preparation by alkaline treatment and detailed characterisation of empty hepatitis B virus core particles for vaccine and gene therapy applications A Strods, V Ose-Klinklāva, J Bogans, I Cielēns, G Kalniņš, I Radoviča-Spalviņa, A Kazāks, P Pumpēns, R Renhofa , Scientific Reports, 5, art. no. 11639 , 2015
The Hepatitis B Virus Core Variants that Expose Foreign C-Terminal Insertions on the Outer Surface of Virus-Like Particles A Dišlers, D Skrastiņa, R Renhofa, I Petrovskis, V Ose-Klinklāva, I Liekniņa, J Jansons, P Pumpēns, I Sominska , Molecular Biotechnology, 57 (11-12), pp. 1038-1049 , 2015
Mosaic RNA phage VLPs carrying domain III of the West Nile virus E protein. I Cielēns, L Jackeviča, A Strods, A Kazāks, V Ose-Klinklāva, J Bogans, P Pumpēns, R Renhofa , FEBS J. 281(11):2613-22 , 2014
Hepatitis C virus hypervariable region 1 variants presented on hepatitis B virus capsid-like particles induce cross-neutralizing antibodies. Lange M, Fiedler M, Bankwitz D, Osburn W, Viazov S, Brovko O, Zekri AR, Khudyakov Y, Nassal M, P Pumpēns, Pietschmann T, Timm J, Roggendorf M, Walker A , PLoS One. 9(7):e102235 , 2014
Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments. R Ranka, I Petrovskis, I Sominska, J Bogans, R Brūvere, I Akopjana, V Ose-Klinklāva, I Timofējeva, K Brangulis, P Pumpēns, V Baumanis , Nanomedicine. Jan;9(1):65-73. , 2013
A VLP library of C-terminally truncated Hepatitis B core proteins: correlation of RNA encapsidation with a Th1/Th2 switch in the immune responses of mice. I Sominska, D Skrastiņa, I Petrovskis, A Dišlers, I Bērza, M Mihailova, J Jansons, I Akopjana, I Stahovska, D Dreiliņa, V Ose-Klinklāva, P Pumpēns , PLoS One. Sep 23;8(9):e75938. , 2013
Chimeric derivatives of hepatitis B virus core particles carrying major epitopes of the rubella virus e1 glycoprotein. D Skrastiņa, I Petrovskis, Petraityte R, I Sominska, V Ose-Klinklāva, I Liekniņa, J Bogans, Sasnauskas K, P Pumpēns , Clin Vaccine Immunol. Nov;20(11):1719-28. , 2013
Posttranslational modifications and secretion efficiency of immunogenic hepatitis B virus L protein deletion variants. B Niedre-Otomere, A Bogdanova-Jātniece, R Brūvere, V Ose-Klinklāva, Gerlich WH, P Pumpēns, Glebe D, T Kozlovska , Virol J. Feb 25;10:63. , 2013
Development of virus-like particle technology from small highly symmetric to large complex virus-like particle structures. Pushko P, P Pumpēns, E Grēns , Intervirology. 56(3):141-65. , 2013
Recombinant Semliki Forest virus vectors encoding hepatitis B virus small surface and pre-S1 antigens induce broadly reactive neutralizing antibodies B Niedre-Otomere, A Bogdanova-Jātniece, D Skrastiņa, A Zajakina, R Brūvere, V Ose-Klinklāva, Gerlich WH, Garoff H, P Pumpēns, Glebe D, T Kozlovska , Journal of Viral Hepatitis, May 17 , 2012
Highly efficient production of phosphorylated hepatitis B core particles in yeast Pichia pastoris. J Freivalds, A Dišlers, V Ose-Klinklāva, P Pumpēns, K Tārs, A Kazāks , Protein Expr Purif. 75(2):218-24. , 2011
Potato Virus Y-Like Particles as a New Carrier for the Presentation of Foreign Protein Stretches I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns, A Zeltiņš , Mol Biotechnol. Dec 14. [Epub ahead of print]. , 2011
N-terminal myristoylation - dependent masking of neutralizing epitopes in the preS1 attachment site of hepatitis B virus Bremer CM, I Sominska, D Skrastiņa, P Pumpēns, Wahed AA EI, Beutling U, Frank R, Fritz HJ, Hunsmann G, Gerlich WH, Glebe D , J Hepatol. Jul;55(1):29-37. , 2011
Local
HBV genoma mutācijas ar lamivudīnu ārstētiem B vīrushepatīta pacientiem Latvijā. D Legzdiņa, Keišs J, G Varblane, I Sominska, Arša F, Davidjuka I, Sondore V, Vīksna L, Rozentāle B, P Pumpēns , RSU Zinātniskie raksti 208-15 , 2009
HCV genoma izmaiņas pretvīrusu terapijas gaitā hroniskas HCV infekcijas gadījumā. G Varblane, J Jansons, I Sominska, Sondore V, Jēruma A, Arša F, Keišs J, Rozentāle B, Vīksna L, P Pumpēns , RSU Zinātniskie raksti 216-23 , 2009
Attenuation spectrum of self-assembled system: semiconductor and virus-like nanoparticles Dekhtyar Yu, Kacanovska A, Mezinskis G, P Pumpēns, R Renhofa , Biomedical Engineering, Proceedings of International Konference. Kaunas University of Technology. Technologija 169-171 , 2008
“Is vaccine against HCV possible?” M Mihailova, I Sominska, Viazov S, P Pumpēns , Acta Universitatis Latviensis 723: 123-43 , 2007
Tumor-asociēto antigēnu inkorporācija vīrusveidīgās daļiņās. Balmaks R, J Freivalds, P Pumpēns, A Kazāks , Acta Universitatis Latviensis 712: 76-9 , 2007
Hepatitis C virus molecular epidemiology in Latvia. J Jansons, G Varblane, I Sominska, P Pumpēns , Acta Universitatis Latviensis 676: 65-70 , 2004
Synthesis of all Hepatitis B structural proteins in Semliki Forest virus expression system. T Kozlovska, A Zajakina, V Ose-Klinklāva, R Brūvere, Alekseeva J, P Pumpēns, Garoff H , Acta Universitatis Latviensis 676: 39-51 , 2004
Subcellular localisation of internally deleted HBV core proteins. R Brūvere, Braun S, A Zajakina, Aleksejeva J, Sharipo A, Meisel H, P Pumpēns, T Kozlovska , Acta Universitatis Latviensis 676: 53-64 , 2004
Structural features of hepatitis B virus from longterm immunosuppressed patients in Latvia. M Mihailova, I Sominska, J Jansons, Rozentals R, P Pumpēns , Proc. Latvian Acad. Sci. 57: 158-163 , 2003
Gene therapy, what it is? P Pumpēns, V Baumanis , Latvijas Ārsts 1: 37-40 , 2001
Vaccines and other immunobiological tools today and tomorrow. P Pumpēns, E Grēns , Latvijas Ārsts 10: 28-32 , 2001
Gene therapy, real success V Baumanis, P Pumpēns , Latvijas Ārsts 3:40-43 , 2001
Specific diagnostics of viral hepatitis B. Current problems. Viksna L, I Sominska, T Kozlovska, J Jansons, P Pumpēns, Arsa F, Rozentale B, Sondore V , LMA Vestis 210-213 , 2000
Display vectors. I. Hepatitis B core particle as a display moiety. Borisova G, Borschukova O, D Skrastiņa, Mezule G, A Dišlers, I Petrovskis, V Ose-Klinklāva, Gusars I, P Pumpēns, E Grēns , Proc. Latvian Acad. Sci. 51:1-7 , 1997
Display vectors. II. Recombinant capsid of RNA bacteriophage Qb as a display moiety. T Kozlovska, I Cielēns, I Vasiļjeva, Bundule M, Strelnikova A, A Kazāks, A Dišlers, D Dreiliņa, V Ose-Klinklāva, Gusars I, P Pumpēns , Proc. Latvian Acad. Sci. 51: 8-12 , 1997
Patents
Paņēmiens tukšu un ar nukleīnskābēm pildītu hepatīta B vīrusa core-proteīna kapsīdu iegūšanai I Liekniņa, I Petrovskis, I Sominska, J Bogans, I Akopjana, P Pumpēns, A Dišlers , LR patenta pieteikuma nr. P-14-81 , 2015
Modificēti HBV core nanokonteineri kā universāla platforma bioloģiska materiāla eksponēšanai R Renhofa, I Cielēns, A Strods, G Kalniņš, D Priede, V Ose-Klinklāva, P Pumpēns , LR patents LV 15034 , 2014
Paņēmiens izvēlēta materiāla piešūšanai HBV core proteīna veidotajām nanodaļiņām R Renhofa, A Kazāks, A Dišlers, L Jackeviča, V Ose-Klinklāva, P Pumpēns , LR patents LV 15033 , 2014
Anti-HCV individuālas terapeitiskas vakcīnas prototips I Sominska, J Jansons, P Pumpēns, I Petrovskis, D Skrastiņa, G. Sudmale , LR patents LV 14987 , 2013
Paņēmiens hepatīta B vīrusa infekcijas modeļa izveidošanai A Zajakina, U Bērziņš, T Kozlovska, P Pumpēns, D Legzdiņa, Sondore V., Keišs J., Vīksna L., I Sominska, Arša F. , LR patents LV 14760 , 2013
Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (8574564) Renner WA, Bachmann MF, I Cielēns, Coester CJ, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Yu , United States Patent 8574564 , 2013
Immunostimulatory nucleic acid packaged particles for the treatment of hypersenstivity (AU2013/204626 A1) Renner W, Bachmann M, I Cielēns, Coester C, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Yu , Australian patent application AU2013/204626 A1 , 2013
Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (AU 2006/325225 B2) R Renhofa, Manolova V, P Pumpēns, Fuchs S, Bachmann M, Tissot A, Coester C, Zou Yu, Dietmeier K, Renner W, Maurer P, I Cielēns, Proba K, Kinzler M , Australian patent AU 2006/325225 B2 , 2013
Ekspresijas sistēma HBc-preS1 vīrusveidīgo daļiņu iegūšanai A Dišlers, I Petrovskis, I Liekniņa, I Bērza, J Bogans, I Akopjana, I Sominska, P Pumpēns , LR patents LV 14945 , 2013
Molecular antigen arrays using a virus like particle derived from the ap205 coat protein. Bachmann M, Tissot A, P Pumpēns, I Cielēns, R Renhofa , EP1532167 (B1); HK1078880 (A1) , 2012
MOLECULAR ANTIGEN ARRAY Bachmann M, Tissot A, P Pumpēns, I Cielēns, R Renhofa , Japanese Patent JP2011087580, Application Number: JP2010000248015 , 2011
Packaging of immunostimulatory substances into virus-like particles: method of preparation and use Bachmann MF, Storni T, Maurer P, Tissot A, Schwarz K, Meijerink E, Lipowsky G, P Pumpēns, I Cielēns, R Renhofa , EP2196217 (A1); US2012301499 (A1) , 2010; 2012
Heterologas proteīnu sekvences eksponējošu kartupeļu vīrusam PVY līdzīgo daļiņu iegūšana un izmantošana. A Zeltiņš, I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns , LR patents Nr. 14204 , 2010
Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (US2010/0303846 A1) Renner W, Bachmann MF, I Cielēns, Coester C, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Y, Proba K, Kinzler M , US2010/0303846 A1 , 2010
Molecular Antigen Arrays. Bachmann MF, Tissot A, P Pumpēns, I Cielēns, R Renhofa , US 2010/0092508 A1 , 2010
Monographs
Construction of novel vaccines on the basis of the virus-like particles: Hepatitis B virus proteins as vaccine carriers. /in "Medicinal Protein Engineering. " pp. 205-248. P Pumpēns, Ulrich R, Sasnauskas K, A Kazāks, V Ose-Klinklāva, E Grēns , CRC Press, Taylor & Francis Group, Boca Raton London, New York , 2009
Artificial genes for chimeric virus-like particles. /in "Artificial DNA: Methods and Applications" pp. 249-327 P Pumpēns, E Grēns , CRC Press LLC, Boca Raton , 2002
Conference thesis
Hepatitis B virus nucleocapsid particles produced in eukaryotic cells: Properties and purification K Spunde, A.Eihentale, A Zajakina, T Kozlovska, P Pumpēns , European Biotechnology Congress 2014 , 2014
Successful creation of chimeric virus-like particles maintaining fibronectin-binding property from B.burgdorferi BBK32 protein R Ranka, I Petrovskis, I Sominska, K Brangulis, P Pumpēns, V Baumanis , XII International Jena Symposium on Tick-borne Diseases. Weimar, Vācija , 2013
Individualized Anti-HCV/HBV Vaccine Prototype. I Sominska, D Skrastiņa, I Petrovskis, V Ose-Klinklāva, I Akopjana, I Stahovska, P Pumpēns , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
Presentation of individual Hepatitis C Hipervariable region. G Varblane, I Sominska, I Petrovskis, V Ose-Klinklāva, Sondore V, Arša F, P Pumpēns , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
Packaging of Nonmethylated CG motifs into Virus-Like Particles Bearing Hepatitis C Virus (HCV) Nonstructural Protein 3 Sequences for Induction of Vigorous HCV Specific Cytotoxic T Cell Response M Mihailova, A Dovbenko, J Bogans, I Petrovskis, V Ose-Klinklāva, I Sominska, P Pumpēns , 4th Annual Proteins Congress & Vaccines Congress, London, UK , 2011
Hepatitis C virus vaccine candidates from chimeric hepatitis B core virus-like particles carrying different fragments of HCV non-structural protein 3 M Mihailova, A Dovbenko, J Bogans, A. Walker, V Ose-Klinklāva, I Sominska, S. Viazov, P Pumpēns , 24th International Conference on Antiviral Research,Sofia, Bulgaria , 2011
A new vaccine strategy for HCV: presentation of hepatitis C virus hypervariable region 1 on HBV capsid-like particles. Milena Lange, Sergei Viazov, Olena Brovko, Hanaa M. Alam El-Din, Nehal Hussien, Yuri Khudyakov, Michael Nassal,, P Pumpēns, Michael Roggendorf, Abdel-RahmanZekri, Andreas Walker , 18th International Symposium on Hepatitis C Virus and Related Viruses, Seattle, Washington, USA, P3.10, p142 , 2011
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EURAXESS
Mājas lapas izstrādi finansēja ERAF 2.1.1.2. aktivitātes projekts Nr. 2010/0196/2DP/2.1.1.2.0/10/APIA/VIAA/004 "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".
