About us

Pauls Pumpēns , Dr. habil. biol.

E-mail: paul@biomed.lu.lv

Groups

• Pauls

Projects

• Fine functional structure of hepatitis B core particles: stable expression in eukaryotic cell lines, purification, structural peculiarities, and immunological evaluation
• Vector-borne Risks for Europe: Risk assessment and control of West Nile and Chikungunya virus (VECTORIE)
• Molecular epidemiology of hepatitis B anc C viruses for the development of individualised therapeutic vaccines
• The molecular effects of quantitative transcriptiion factor alterations in cancer cells in vitro
• Introduction of molecular pinciples in viral hepatitis diagnostics, prognostics, treatment and prevalence restriction
• Artificial virus-like particles as a platform for novel vaccine, gene therapy and diagnostic tools
• Novel chimeric vaccine prototypes: West nile virus
• Ration design and standardized evalution of novel genetic vaccines using West Nile virus as a paradigm
• Construction of melanoma chimeric vaccine candidates
• Combined immune and gene therapy for chronic hepatitis
• Molecular mechanisms of genetic variability and persistence of viral hepatitis
• Malaria vaccine
• Novel VLP model: expression and X-ray structure of coat protein and chimeras of Acinetobacter RNA phage AP205
• Hematological malignancies in children and clinical properties in influence of combined chronical viral infection
• New generation diagnostic of sexually transmitted diseases
• Artificial chimeric proteins as diagnostic, vaccine, and gene therapy
• Molecular monitoring and pathological role of HCV, HGV and altered HBV genomes in Baltic region
• Bivalent hantavirus vaccine for Europe
• Universal Cloning and Expression System by Means of Protease Mutants
• Spatial Structure of Chimeric Capsids Based on RNA Phage Coats
• Construction of E.coli Mutants - Overproducers of Chimeric Proteins RNA Phage Coats
• Molecular Monitoring of HBV and HCV Infection in Latvia: Genomic Variations of HBV and HCV in Presence and Absence of HIV Infection

Publications

• International

• The True Story and Advantages of RNA Phage Capsids as Nanotools P Pumpēns, R Renhofa, A Dišlers, T Kozlovska, V Ose-Klinklāva, Pushko, P., K Tārs, E Grēns, Bachmann, M.F. , Intervirology, pp. 74-110 , 2016
• The true story and advantages of the famous Hepatitis B virus core particles: Outlook 2016 P Pumpēns, E Grēns , Molecular Biology, 50 (4), pp. 489-509 , 2016
• Preparation by alkaline treatment and detailed characterisation of empty hepatitis B virus core particles for vaccine and gene therapy applications A Strods, V Ose-Klinklāva, J Bogans, I Cielēns, G Kalniņš, I Radoviča-Spalviņa, A Kazāks, P Pumpēns, R Renhofa , Scientific Reports, 5, art. no. 11639 , 2015
• The Hepatitis B Virus Core Variants that Expose Foreign C-Terminal Insertions on the Outer Surface of Virus-Like Particles A Dišlers, D Skrastiņa, R Renhofa, I Petrovskis, V Ose-Klinklāva, I Liekniņa, J Jansons, P Pumpēns, I Sominska , Molecular Biotechnology, 57 (11-12), pp. 1038-1049 , 2015
• Mosaic RNA phage VLPs carrying domain III of the West Nile virus E protein. I Cielēns, L Jackeviča, A Strods, A Kazāks, V Ose-Klinklāva, J Bogans, P Pumpēns, R Renhofa , FEBS J. 281(11):2613-22 , 2014
• Hepatitis C virus hypervariable region 1 variants presented on hepatitis B virus capsid-like particles induce cross-neutralizing antibodies. Lange M, Fiedler M, Bankwitz D, Osburn W, Viazov S, Brovko O, Zekri AR, Khudyakov Y, Nassal M, P Pumpēns, Pietschmann T, Timm J, Roggendorf M, Walker A , PLoS One. 9(7):e102235 , 2014
• Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments. R Ranka, I Petrovskis, I Sominska, J Bogans, R Brūvere, I Akopjana, V Ose-Klinklāva, I Timofējeva, K Brangulis, P Pumpēns, V Baumanis , Nanomedicine. Jan;9(1):65-73. , 2013
• A VLP library of C-terminally truncated Hepatitis B core proteins: correlation of RNA encapsidation with a Th1/Th2 switch in the immune responses of mice. I Sominska, D Skrastiņa, I Petrovskis, A Dišlers, I Bērza, M Mihailova, J Jansons, I Akopjana, I Stahovska, D Dreiliņa, V Ose-Klinklāva, P Pumpēns , PLoS One. Sep 23;8(9):e75938. , 2013
• Chimeric derivatives of hepatitis B virus core particles carrying major epitopes of the rubella virus e1 glycoprotein. D Skrastiņa, I Petrovskis, Petraityte R, I Sominska, V Ose-Klinklāva, I Liekniņa, J Bogans, Sasnauskas K, P Pumpēns , Clin Vaccine Immunol. Nov;20(11):1719-28. , 2013
• Posttranslational modifications and secretion efficiency of immunogenic hepatitis B virus L protein deletion variants. B Niedre-Otomere, A Bogdanova-Jātniece, R Brūvere, V Ose-Klinklāva, Gerlich WH, P Pumpēns, Glebe D, T Kozlovska , Virol J. Feb 25;10:63. , 2013
• Development of virus-like particle technology from small highly symmetric to large complex virus-like particle structures. Pushko P, P Pumpēns, E Grēns , Intervirology. 56(3):141-65. , 2013
• Recombinant Semliki Forest virus vectors encoding hepatitis B virus small surface and pre-S1 antigens induce broadly reactive neutralizing antibodies B Niedre-Otomere, A Bogdanova-Jātniece, D Skrastiņa, A Zajakina, R Brūvere, V Ose-Klinklāva, Gerlich WH, Garoff H, P Pumpēns, Glebe D, T Kozlovska , Journal of Viral Hepatitis, May 17 , 2012
• Highly efficient production of phosphorylated hepatitis B core particles in yeast Pichia pastoris. J Freivalds, A Dišlers, V Ose-Klinklāva, P Pumpēns, K Tārs, A Kazāks , Protein Expr Purif. 75(2):218-24. , 2011
• Potato Virus Y-Like Particles as a New Carrier for the Presentation of Foreign Protein Stretches I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns, A Zeltiņš , Mol Biotechnol. Dec 14. [Epub ahead of print]. , 2011
• N-terminal myristoylation - dependent masking of neutralizing epitopes in the preS1 attachment site of hepatitis B virus Bremer CM, I Sominska, D Skrastiņa, P Pumpēns, Wahed AA EI, Beutling U, Frank R, Fritz HJ, Hunsmann G, Gerlich WH, Glebe D , J Hepatol. Jul;55(1):29-37. , 2011

• Local

• HBV genoma mutācijas ar lamivudīnu ārstētiem B vīrushepatīta pacientiem Latvijā. D Legzdiņa, Keišs J, G Varblane, I Sominska, Arša F, Davidjuka I, Sondore V, Vīksna L, Rozentāle B, P Pumpēns , RSU Zinātniskie raksti 208-15 , 2009
• HCV genoma izmaiņas pretvīrusu terapijas gaitā hroniskas HCV infekcijas gadījumā. G Varblane, J Jansons, I Sominska, Sondore V, Jēruma A, Arša F, Keišs J, Rozentāle B, Vīksna L, P Pumpēns , RSU Zinātniskie raksti 216-23 , 2009
• Attenuation spectrum of self-assembled system: semiconductor and virus-like nanoparticles Dekhtyar Yu, Kacanovska A, Mezinskis G, P Pumpēns, R Renhofa , Biomedical Engineering, Proceedings of International Konference. Kaunas University of Technology. Technologija 169-171 , 2008
• “Is vaccine against HCV possible?” M Mihailova, I Sominska, Viazov S, P Pumpēns , Acta Universitatis Latviensis 723: 123-43 , 2007
• Tumor-asociēto antigēnu inkorporācija vīrusveidīgās daļiņās. Balmaks R, J Freivalds, P Pumpēns, A Kazāks , Acta Universitatis Latviensis 712: 76-9 , 2007
• Hepatitis C virus molecular epidemiology in Latvia. J Jansons, G Varblane, I Sominska, P Pumpēns , Acta Universitatis Latviensis 676: 65-70 , 2004
• Synthesis of all Hepatitis B structural proteins in Semliki Forest virus expression system. T Kozlovska, A Zajakina, V Ose-Klinklāva, R Brūvere, Alekseeva J, P Pumpēns, Garoff H , Acta Universitatis Latviensis 676: 39-51 , 2004
• Subcellular localisation of internally deleted HBV core proteins. R Brūvere, Braun S, A Zajakina, Aleksejeva J, Sharipo A, Meisel H, P Pumpēns, T Kozlovska , Acta Universitatis Latviensis 676: 53-64 , 2004
• Structural features of hepatitis B virus from longterm immunosuppressed patients in Latvia. M Mihailova, I Sominska, J Jansons, Rozentals R, P Pumpēns , Proc. Latvian Acad. Sci. 57: 158-163 , 2003
• Gene therapy, what it is? P Pumpēns, V Baumanis , Latvijas Ārsts 1: 37-40 , 2001
• Vaccines and other immunobiological tools today and tomorrow. P Pumpēns, E Grēns , Latvijas Ārsts 10: 28-32 , 2001
• Gene therapy, real success V Baumanis, P Pumpēns , Latvijas Ārsts 3:40-43 , 2001
• Specific diagnostics of viral hepatitis B. Current problems. Viksna L, I Sominska, T Kozlovska, J Jansons, P Pumpēns, Arsa F, Rozentale B, Sondore V , LMA Vestis 210-213 , 2000
• Display vectors. I. Hepatitis B core particle as a display moiety. Borisova G, Borschukova O, D Skrastiņa, Mezule G, A Dišlers, I Petrovskis, V Ose-Klinklāva, Gusars I, P Pumpēns, E Grēns , Proc. Latvian Acad. Sci. 51:1-7 , 1997
• Display vectors. II. Recombinant capsid of RNA bacteriophage Qb as a display moiety. T Kozlovska, I Cielēns, I Vasiļjeva, Bundule M, Strelnikova A, A Kazāks, A Dišlers, D Dreiliņa, V Ose-Klinklāva, Gusars I, P Pumpēns , Proc. Latvian Acad. Sci. 51: 8-12 , 1997

• Patents

• Paņēmiens tukšu un ar nukleīnskābēm pildītu hepatīta B vīrusa core-proteīna kapsīdu iegūšanai I Liekniņa, I Petrovskis, I Sominska, J Bogans, I Akopjana, P Pumpēns, A Dišlers , LR patenta pieteikuma nr. P-14-81 , 2015
• Modificēti HBV core nanokonteineri kā universāla platforma bioloģiska materiāla eksponēšanai R Renhofa, I Cielēns, A Strods, G Kalniņš, D Priede, V Ose-Klinklāva, P Pumpēns , LR patents LV 15034 , 2014
• Paņēmiens izvēlēta materiāla piešūšanai HBV core proteīna veidotajām nanodaļiņām R Renhofa, A Kazāks, A Dišlers, L Jackeviča, V Ose-Klinklāva, P Pumpēns , LR patents LV 15033 , 2014
• Anti-HCV individuālas terapeitiskas vakcīnas prototips I Sominska, J Jansons, P Pumpēns, I Petrovskis, D Skrastiņa, G. Sudmale , LR patents LV 14987 , 2013
• Paņēmiens hepatīta B vīrusa infekcijas modeļa izveidošanai A Zajakina, U Bērziņš, T Kozlovska, P Pumpēns, D Legzdiņa, Sondore V., Keišs J., Vīksna L., I Sominska, Arša F. , LR patents LV 14760 , 2013
• Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (8574564) Renner WA, Bachmann MF, I Cielēns, Coester CJ, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Yu , United States Patent 8574564 , 2013
• Immunostimulatory nucleic acid packaged particles for the treatment of hypersenstivity (AU2013/204626 A1) Renner W, Bachmann M, I Cielēns, Coester C, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Yu , Australian patent application AU2013/204626 A1 , 2013
• Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (AU 2006/325225 B2) R Renhofa, Manolova V, P Pumpēns, Fuchs S, Bachmann M, Tissot A, Coester C, Zou Yu, Dietmeier K, Renner W, Maurer P, I Cielēns, Proba K, Kinzler M , Australian patent AU 2006/325225 B2 , 2013
• Ekspresijas sistēma HBc-preS1 vīrusveidīgo daļiņu iegūšanai A Dišlers, I Petrovskis, I Liekniņa, I Bērza, J Bogans, I Akopjana, I Sominska, P Pumpēns , LR patents LV 14945 , 2013
• Molecular antigen arrays using a virus like particle derived from the ap205 coat protein. Bachmann M, Tissot A, P Pumpēns, I Cielēns, R Renhofa , EP1532167 (B1); HK1078880 (A1) , 2012
• MOLECULAR ANTIGEN ARRAY Bachmann M, Tissot A, P Pumpēns, I Cielēns, R Renhofa , Japanese Patent JP2011087580, Application Number: JP2010000248015 , 2011
• Packaging of immunostimulatory substances into virus-like particles: method of preparation and use Bachmann MF, Storni T, Maurer P, Tissot A, Schwarz K, Meijerink E, Lipowsky G, P Pumpēns, I Cielēns, R Renhofa , EP2196217 (A1); US2012301499 (A1) , 2010; 2012
• Heterologas proteīnu sekvences eksponējošu kartupeļu vīrusam PVY līdzīgo daļiņu iegūšana un izmantošana. A Zeltiņš, I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns , LR patents Nr. 14204 , 2010
• Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity (US2010/0303846 A1) Renner W, Bachmann MF, I Cielēns, Coester C, Dietmeier K, Fuchs S, Manolova V, Maurer P, P Pumpēns, R Renhofa, Tissot A, Zou Y, Proba K, Kinzler M , US2010/0303846 A1 , 2010
• Molecular Antigen Arrays. Bachmann MF, Tissot A, P Pumpēns, I Cielēns, R Renhofa , US 2010/0092508 A1 , 2010

• Monographs

• Construction of novel vaccines on the basis of the virus-like particles: Hepatitis B virus proteins as vaccine carriers. /in "Medicinal Protein Engineering. " pp. 205-248. P Pumpēns, Ulrich R, Sasnauskas K, A Kazāks, V Ose-Klinklāva, E Grēns , CRC Press, Taylor & Francis Group, Boca Raton London, New York , 2009
• Artificial genes for chimeric virus-like particles. /in "Artificial DNA: Methods and Applications" pp. 249-327 P Pumpēns, E Grēns , CRC Press LLC, Boca Raton , 2002

• Conference thesis

• Hepatitis B virus nucleocapsid particles produced in eukaryotic cells: Properties and purification K Spunde, A.Eihentale, A Zajakina, T Kozlovska, P Pumpēns , European Biotechnology Congress 2014 , 2014
• Successful creation of chimeric virus-like particles maintaining fibronectin-binding property from B.burgdorferi BBK32 protein R Ranka, I Petrovskis, I Sominska, K Brangulis, P Pumpēns, V Baumanis , XII International Jena Symposium on Tick-borne Diseases. Weimar, Vācija , 2013
• Individualized Anti-HCV/HBV Vaccine Prototype. I Sominska, D Skrastiņa, I Petrovskis, V Ose-Klinklāva, I Akopjana, I Stahovska, P Pumpēns , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
• Presentation of individual Hepatitis C Hipervariable region. G Varblane, I Sominska, I Petrovskis, V Ose-Klinklāva, Sondore V, Arša F, P Pumpēns , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
• Packaging of Nonmethylated CG motifs into Virus-Like Particles Bearing Hepatitis C Virus (HCV) Nonstructural Protein 3 Sequences for Induction of Vigorous HCV Specific Cytotoxic T Cell Response M Mihailova, A Dovbenko, J Bogans, I Petrovskis, V Ose-Klinklāva, I Sominska, P Pumpēns , 4th Annual Proteins Congress & Vaccines Congress, London, UK , 2011
• Hepatitis C virus vaccine candidates from chimeric hepatitis B core virus-like particles carrying different fragments of HCV non-structural protein 3 M Mihailova, A Dovbenko, J Bogans, A. Walker, V Ose-Klinklāva, I Sominska, S. Viazov, P Pumpēns , 24th International Conference on Antiviral Research,Sofia, Bulgaria , 2011
• A new vaccine strategy for HCV: presentation of hepatitis C virus hypervariable region 1 on HBV capsid-like particles. Milena Lange, Sergei Viazov, Olena Brovko, Hanaa M. Alam El-Din, Nehal Hussien, Yuri Khudyakov, Michael Nassal,, P Pumpēns, Michael Roggendorf, Abdel-RahmanZekri, Andreas Walker , 18th International Symposium on Hepatitis C Virus and Related Viruses, Seattle, Washington, USA, P3.10, p142 , 2011

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