About us

Jānis Kloviņš , Dr. biol., Assoc. prof.

Director
Phone: +37167808003
E-mail: klovins@biomed.lu.lv

Groups

• J. Klovins lab

Projects

• Molecular markers of pituitary tumor development, progression and therapy response
• Investigation of interplay between multiple determinants influencing response to metformin: search for reliable predictors for efficacy of type 2 diabetes therapy
• Promoting scientific excellence and institutional development of Latvian Biomedical Research and Study Centre
• Development of novel in vitro tests for diagnostics and prognostics of individualized therapies of tumors and mitochondrial disease treatment
• Research and development of novel diagnostic methods for genetic and immunologic testing
• Molecular Mechanisms, Pharmacogenetics and New Medicines for Treatment of Diabetes and Cardiovascular Complications
• Preparatory visit for project “Genetics and functionality of pituitary tumor development, progression and therapy responce"
• BBMRI- Large Prospective Cohorts (LPC)
• The Development of the Scientific Infrastructure of the Pharmaceutical and Biomedical Research Centre of National Importance
• Development and validation of novel DNA tests for diagnosis applicable in clinical practice
• Development of novel G protein coupled receptor assay systems for bioactive compound monitoring
• Pharmacogenetics of diabetes and cardiovascular diseases, testing of therapeutic target-receptors
• The genetics of metabolic and endocrine disease
• Genotyping and sequencing service
• Role of new G-protein coupled receptors on adipogenesis, lipolysis and adipokine secretion in white adipocytes: functional and genetic studies.
• Definition of pharmacogenetic markers for the prediction of efficacy and side-effects of metformin therapy of type 2 diabetes
• The molecular foudation for individual variation in body weight regulation
• Creation of united accesable data base for researchers containing life span and life quality influencing pathologies and its' risk factor prevalence in population of Latvia
• Development of set of activities for modern early diagnostics, profilaxis and therapy for children diseases causinf early mortality and disability
• G protein coupled receptor ligands - potentially novel therapeutics
• Development and approbation of new genetic diagnostic tests in Latvia
• Development of molecular genetic analysis methods and it's application in genetic research: Service of Latvian Genome centre.
• Research of genetic factors of metabolism and homeostasis regulation
• In cenrtal nervous system localized G protein coupled receptors - structural and functional research and serearch of novel ligands
• Research of melanocortine receptor structures, functions and molecular evolution
• Analysis of pituitary melanocortine system involved gene polymorphysms and its' association with human energy homeostasis alteration in Latvia

Publications

• International

• Variants in Pharmacokinetic Transporters and Glycaemic Response to Metformin: A MetGen Meta-Analysis Dujic, T., Zhou, K., Yee, S.W., van Leeuwen N., de Keyser, C.E., Javorský, M., Goswami, S., L Zaharenko, Marie, M., Christensen, H., Out, M., Tavendale, R., Kubo, M., Hedderson, M.M., van der Heijden, A.A., Klimčáková, L., V Pīrāgs, Kooy, A., Brøsen, K., J Kloviņš, Semiz, S., Tkáč, I., Stricker, B.H., Palmer, C.N., 't Hart, L.M., Giacomini, K.M., Pearson, E.R. , Clinical Pharmacology & Therapeutics , 2016
• Single nucleotide polymorphisms in the intergenic region between metformin transporter OCT2 and OCT3 genes are associated with short-term response to metformin monotherapy in type 2 diabetes mellitus patients L Zaharenko, I Kalniņa, Geldnere, K., Konrade, I., Grinberga, S., Židzik ,J., Javorský, M., Lejnieks, A., L Ņikitina-Zaķe, D Fridmanis, R Pečulis, I Radoviča-Spalviņa, Hartmane, D., Pugovics, O., Tkáč, I., Klimčáková, L., V Pīrāgs, J Kloviņš , European Journal of Endocrinology , 2016
• Variation in the glucose transporter gene SLC2A2 is associated with glycemic response to metformin Zhou, K., Yee, S.W., Seiser, E.L., Van Leeuwen, N., Tavendale, R., Bennett, A.J., Groves, C.J., Coleman, R.L., Van Der Heijden, A.A., Beulens, J.W., De Keyser, C.E., L Zaharenko, ., Rotroff, D.M., Out, M., Jablonski, K.A., Chen, L., Javorský, M., Zidzik, J., Levin, A.M., Keoki Williams, L., Dujic, T., Semiz, S., Kubo, M., Chien, H.-C., Maeda, S., Witte, J.S., Wu, L., Tká, I., Kooy, A., Van Schaik, R.H.N., Stehouwer, C.D.A., Logie,, J Kloviņš, V Pīrāgs, Hofman, A., Stricker, B.H., Motsinger-Reif, A.A., Wagner, M.J., Innocenti, F., Hart, L.M., Holman, R.R., McCarthy, M.I., Hedderson, M.M., Palmer, C.N.A., Florez, J.C., Giacomini, K.M., Pearson, E.R. , Nature Genetics, 48 (9), pp. 1055-1059 , 2016
• Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas K Megnis, I Mandrika, R Petrovska, Stukens, J., V Rovīte, Balcere, I., Jansone, L.S., R Pečulis, V Pīrāgs, J Kloviņš , Stem Cells International, 2016, art. no. 7103720 , 2016
• Polymorphisms in MEN1 and DRD2 genes are associated with the occurrence and characteristics of pituitary adenomas R Pečulis, Balcere, I., V Rovīte, K Megnis, Valtere, A., Stukens, J., Arnicane, L., L Ņikitina-Zaķe, Lejnieks, A., V Pīrāgs, J Kloviņš , European Journal of Endocrinology, 175 (2), pp. 145-153 , 2016
• Prevalence estimation of celiac disease in the general adult population of Latvia using serology and HLA genotyping Leja M., Shums Z., L Ņikitina-Zaķe, Gavars M., Kikuste I., Milo J., Daugule I., Pahomova J., V Pīrāgs, Dzerve V., J Kloviņš, Erglis A., Norman GL. , United European Gastroenterol J. (2015) Apr;3(2):190-9 , 2015
• LD score regression distinguishes confounding from polygenicity in genome-wide association studies Bulik-Sullivan, B., ..., J Kloviņš, L Ņikitina-Zaķe, … O'Donovan, M.C. , Nature Genetics, 47 (3), pp. 291-295 , 2015
• Modeling Linkage Disequilibrium Increases Accuracy of Polygenic Risk Scores Vilhjálmsson, B.J., ..., J Kloviņš, L Ņikitina-Zaķe, ... Price, A.L. , American Journal of Human Genetics, 97 (4), pp. 576-592. , 2015
• Evaluation of massive parallel sequencing as a diagnostic tool for familial hypercholesterolemia I Radoviča-Spalviņa, Berzins R, Latkovskis G, D Fridmanis, L Ņikitina-Zaķe, Ventins K, Ozola G, Erglis A, J Kloviņš , Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, 69 (1-2), pp. 1-7 , 2015
• Harmonising and linking biomedical and clinical data across disparate data archives to enable integrative cross-biobank research. Spjuth O, Krestyaninova M, Hastings J, Shen HY, Heikkinen J, Waldenberger M, Langhammer A, Ladenvall C, Esko T, Persson MÅ, Heggland J, Dietrich J, Ose S, Gieger C, Ried JS, Peters A, Fortier I, de Geus EJ, J Kloviņš, L Zaharenko, Willemsen G, Hottenga JJ, Litton JE, Karvanen J, Boomsma DI, Groop L, Rung J, Palmgren J, Pedersen NL, McCarthy MI, van Duijn CM, Hveem K, Metspalu A, Ripatti S, Prokopenko I, Harris JR , Eur J Hum Genet. doi: 10.1038 , 2015
• Many obesity-associated SNPs strongly associate with DNA methylation changes at proximal promoters and enhancers Voisin, S., Almén, M.S., Zheleznyakova, G.Y., Lundberg, L., Zarei, S., Castillo, S., Eriksson, F.E., Nilsson, E.K., Blüher, M., Böttcher, Y., Kovacs, P., J Kloviņš, Rask-Andersen, M., Schiöth, H.B. , Genome Medicine, 7 (1), art. no. 103. , 2015
• Replacement of short segments within transmembrane domains of MC2R disrupts retention signal. D Fridmanis, R Petrovska, D Pjanova, Schiöth HB, J Kloviņš , J Mol Endocrinol. 53(2):201-15. , 2014
• The role of common and rare MC4R variants and FTO polymorphisms in extreme form of obesity V Rovīte, R Petrovska, I Vaivade, I Kalniņa, D Fridmanis, L Zaharenko, R Pečulis, V Pīrāgs, Schioth HB, J Kloviņš , Mol Biol Rep. 41(3):1491-500 , 2014
• HFE-related hemochromatosis risk mutations in Latvian population. R Pečulis, B Lāce, Putnina A, L Ņikitina-Zaķe, J Kloviņš , Ann Hematol. 94(2):343-4 , 2014
• Genome-wide analysis reveals DNA methylation markers that vary with both age and obesity. Almén MS, Nilsson EK, Jacobsson JA, I Kalniņa, J Kloviņš, Fredriksson R, Schiöth HB , Gene. 548(1):61-7 , 2014

• Local

• Analysis of Polymorphisms at the Adiponectin Gene Locus in Association with Type 2 Diabetes, Body Mass Index and Cardiovascular. I Kalniņa, Latkovskis G, L Ņikitina-Zaķe, Mackevičs V, R Pečulis, I Kāpa, D Fridmanis, Ērglis A, Pīrāgs V, J Kloviņš , Proc. Latvian Acad. Sci. 63: 174-9 , 2009
• Glucose Metabolism Disorders and Risk Factors of Type 2 Diabetes in 45-74-Years-old Population in Rīga Barengo NC, Misiņa D, Zariņa L, J Kloviņš, Ozola D, Dērveniece A, L Zaharenko, Pīrāgs V. , Proc. Latvian Acad. Sci. 63: 141-6 , 2009
• Incidence of Borrelia Burgdorferi infected ticks in Latvia. V Baumanis, J Kloviņš, R Ranka, Vetcmane-Vetce K, Puzuka A, Lavrinoviča M, Bormane A, Kalniņa V , Proc. Latvian Acad. Sci. 4: 21-29 , 2001
• Incidence of Borrelia Burgdorferi infected ticks in Latvia and their molecular typing. V Baumanis, J Kloviņš, R Ranka, Vetcmane-Vetce K, Puzuka A, Lavrinoviča M, Bormane A, Kalniņa V , Proc. Latvian Acad. Sci. 55: 164-170 , 2001

• Patents

• SET OF OLIGONUCLEOTIDES AND METHOD FOR DETECTION OF FETAL DNA FRACTION IN MATERNAL PLASMA I Radoviča-Spalviņa, V Rovīte, R Pečulis, L Ņikitina-Zaķe, D Fridmanis, J Kloviņš , Europena Patent Office, Application number EP15182851.4 , 2015
• SNP copmosition and method for diagnosing risk for dyslipidemia I Radoviča-Spalviņa, D Fridmanis, L Ņikitina-Zaķe, J Kloviņš , Europena Patent Office EP13192992.9 , 2013
• Viena nukleotīda polimorfismu komplekts paaugstināta vai pazemināta augsta blīvuma lipoproteīna holesterola līmeņa asinīs ģenētiskās predisponētības noteikšanai I Radoviča-Spalviņa, D Fridmanis, L Ņikitina-Zaķe, J Kloviņš , LR patents LV 15022 , 2013
• Rekombinanta himēriska polipeptīda kDNS, kas satur melanokortīna otrā tipa receptora (MC2R) un melanokortīnu receptoru palīgproteīna (MRAP) secības, un tās izmantošana aktīvo vielu testēšanai un terapeitiskiem mērķiem D Fridmanis, J Kloviņš, I Mandrika, R Petrovska, Roga A , LR patents LV 15035 , 2013
• 1,4-Dihidropiridīn-4-IL-piridīnija atvasinājumi kā jauni adenozīna A2A receptora agoallostērisku modulatori Duburs G, Brūvere I., J Kloviņš, R Petrovska, I Mandrika, V Rovīte, Bisenieks E, Uldriķis J, Poikāns J, Vīgante B , LR patents LV 14884 , 2013
• 3’-Aril- un heterilaizvietotās 2-akrilamidocikloheks-1-ēn-karbonskābes kā hidroksikarbonskābju receptoru saimes (HCA2) jauna ligandu grupa Loža E, Bobiļeva O, Bokaldere R, Gailīte V, Kaula I, Ikaunieks M, I Mandrika, R Petrovska, J Kloviņš, Duburs G, Bisenieks E , LR patents LV 14982 , 2013
• Heterociklā aizvietoti[(2-karboksi vai metoksikarbonil)fenilkarbamoil-metil-(vai trimetilēn)]piridīnija vai izohinolīnija bromīdi kā hidroksikarbonskābju receptoru saimes(HCA2) jauna ligandu klase Vīgante B, Luntēna I, Kalme Z, Bisenieks E, Poikāns J, R Petrovska, I Mandrika, J Kloviņš, Loža E, Brūvere I., Duburs G, Uldriķis J , LR patents LV15002 , 2013
• 5-(4-hlorbutil)-pirano[2,3-d]pirimidīn-2,4,7(3H)trions un 5-alkil-2-tiokso-1H-pirano[2,3-d]-pirimidīn-4,7-dioni kā hidroksikarbonskābju receptora HCA2(GPR109A) jauni selektīvi ligandi un sintoni jaunu ligandu iegūšana Vīgande B, Brūvere I.,, Bisenieks E, I Mandrika, R Petrovska, J Kloviņš, Poikāns J, Uldriķis J, Duburs G , LR patents LV 15004 , 2013
• 1,4-Dihidopiridīn-4-il-piridīniju atvasinājumi kā jauni A2A receptora agolallostēriskie modulatori Duburs G, J Kloviņš, Brūvere I., R Petrovska, I Mandrika, V Rovīte, Bisenieks E, Uldriķis J, Poikāns J, Dambrova M, Vilšķērsts R, Vīgante B , LR Patenta pieteikums P-09-19 , 2009

• Conference thesis

• Pharmacokinetics and pharmacogenetics of metformin in 21 healthy participant after 500 mg single-dose administration L Zaharenko, I.Konrāde, A.Lejnieks, V Pīrāgs, D.Hartmane, S.Grinberga, O.Pugovičs, I Radoviča-Spalviņa, R Pečulis, J Kloviņš , 29th Congress of Central European Diabetes Association. Organizator: Central European Diabetes Association Federation of International Danube Symposia on Diabetes mellitus, Jūrmala, Latvija , 2014
• The susceptibility variants of type two diabetes in extremely obese individuals V Rovīte, R Petrovska, I Vaivade, I Kalniņa, D Fridmanis, L Zaharenko, R Pečulis, V Pīrāgs, Helgi B. Schioth, J Kloviņš , 29th Congress of the Federation of the International Danube Symposia on Diabetes mellitus and 9th Congress of Central European Diabetes, Jūrmala, Latvija , 2014
• Genetic variants in SKI, FGFR1, WNT3, IRF6 and BMP4 genes are associated with risk for non-syndromic CL/CLP and/or CP in Latvian population Kempa L, Piekuse I, Akota B, Barkane A, A Krūmiņa, J Stāvusis, J Kloviņš, B Lāce , European Journal of Human Genetics, Volume 21, Suppl. 2. , 2013
• The association of haplotypes in CETP gene with HDL cholesterol levels in Latvian population I Radoviča-Spalviņa, D Fridmanis, I Vaivade, L Ņikitina-Zaķe, J Kloviņš , European School of Genetic Medicine, 2nd course in next generation sequencing, Bertinoro, Italy. Programme and Abstract book, p.34. , 2013
• The association of common SNPs with HDL cholesterol levels in Latvian population I Radoviča-Spalviņa, D Fridmanis, I Vaivade, L Ņikitina-Zaķe, J Kloviņš , European Journal of Human Genetics, Volume 21, Supplement 2, P10.13, p.246. , 2013
• Association of IRF6 gene polymorphisms with nonsyndromic cleft lip with or without cleft palate. Kempa I, Akota B, Barkane A, A Krūmiņa, J Kloviņš, B Lāce , European J Hum Gen, 20 Suppl. 1: p234. , 2012
• Polymorphisms in gyoxalase 1 gene constitute to altered enzyme activity. Konrade I, R Pečulis, Skapare E, J Kloviņš, Dambrova M , 4th International Conference on Quantitative Genetics: Understanding Variation in Complex Traits. Edinburgh, Scotland. P-64. , 2012
• Association of polymorphisms representing obesity-related loci comprising FTO and TMEM18 with type 2 diabetes and age at diagnosis of the disease in the population of Latvia. I Kalniņa, L Zaharenko, I Vaivade, V Rovīte, Jacobsson JA, Almen MS, Schiöth HB, V Pīrāgs, J Kloviņš , European Human Genetics Conference. Nürnberg, Germany. P09-136. , 2012
• Analysis of the MC4R in relation to obesity in population of Latvia. V Rovīte, R Petrovska, I Kalniņa, I Vaivade, D Fridmanis, L Zaharenko, R Pečulis, Schioth HB, J Kloviņš , European Human Genetics Conference. Nürnberg, Germany. P09-098. , 2012
• Common Variants in FTO and TMEM18 Loci Associate with Type 2 Diabetes and BMI in the Population of Latvia. I Kalniņa, L Zaharenko, I Vaivade, V Pīrāgs, Jacobsson JA, Almen MS, Schiöth HB, J Kloviņš , International Congress on Personalized Medicine: Up Close and Personalized. Florence, Italy. P-10. , 2012
• Association of genetic variation in the organic cation transporters OCT1, OCT2 and multidrug and toxin extrusion 1 transporter protein genes with the gastrointestinal side-effects in metformin treated type 2 diabetes patients. L Zaharenko, I Kalniņa, Geldnere K, Bumbure A, Ritenberga R, L Ņikitina-Zaķe, D Fridmanis, I Vaivade, V Pīrāgs, J Kloviņš , International Congress on Personalized Medicine: Up Close and Personalized. Florence, Italy. , 2012
• Melanocortin 4 receptor genetics in defining the morbid obesity in general population: common polymorphisms versus mutations. J Kloviņš, V Rovīte, Schioth H, V Pīrāgs , 15th International and 14th European Congress of Endocrinology. Florence, Italy. P1193. , 2012
• OPTIMED Consortium. Identification of novel polymorphisms associated with efficiency of metformin treatment in type 2 diabetes patients. J Kloviņš, L Zaharenko, Zarina L, V Pīrāgs , ASGH Annual Meeting. Sanfrancisco, USA. Poster 608T. , 2012
• Modulation of inflammatory cytokine gene expression by niacin receptor ligands in LPS-challenged human SGBS adipocytes and THP-1 macrophages. I Mandrika, R Petrovska, J Kloviņš , International Conference in Pharmacology: Targeting cellular regulatory systems. Riga, Latvia. , 2012
• Polymorphisms in FGF1 gene are associated with nonsyndromic cleft lip and palate I Kempa, L Piekuse, J Kloviņš, B Barkane, I Akota, A Krūmiņa, B Lāce , EHG Conference, Eur J Hum Genet, Abstracts, vol. 19, suppl.2, p.307 , 2011

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